doi: 10.2337/diab.45.8.1024
Risk factors include occupations requiring prolonged standing, being overweight, having flat feet or high arches, tight calf muscles, and sudden increases in physical activity
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The program of qPCR reaction included 95 C for 10 min, 40 cycles of 95 C for 5 s and 60 C for 15 s
The shortage of RF could also be a consequence of the use of some drugs, alcohol consumption, increased need of RF due to physiological conditions like pregnancy or breastfeeding or childhood etc
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