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In the study above, the authors note that more drugs [from postauricular injection] concentrated in the inner ear for longer therapeutic time, and less systemic delivery implied less risk of side effects. Practical Considerations: Injection Scheduling Protocol typically involves multiple injections over 12 weeks

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No severe adverse events have been reported in published NAD+/NADH trials

This maintenance role, where peptides keep the skin stable between conventional treatment courses, may be the most practical near-term application

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After centrifugation, methanol-containing proteins were precipitated with cold trichloroacetic acid (TCA) and reduced with 500 mM sodium borohydride in 0.2 M borate buffer (pH 9.2) after overnight incubation at room temperature