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[PubMed] [Google Scholar] 31.Nelson SD, Mitchell JR, Timbrell JA, Snodgrass WR, Corcoran GB

Development and characterization of glutamyl-protected N-hydroxyguanidines as reno-active nitric oxide donor drugs with therapeutic potential in acute renal failure

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Labs use methods like the Paglia and Valentine assay, where samples react with substrates like cumene hydroperoxide, and NADPH consumption is tracked spectrophotometrically

NR mildly but significantly decreased DCF fluorescence at 1 M, while, increasing DCF fluorescence at 10 M in the presence of mitochondria and NADH