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The ARRIVE 2.0 checklist (Animal Research: Reporting of In Vivo Experiments) has become standard for any publication and covers 21 items (design, sample size, randomisation, blinding, statistics, ethics)

It was advocated that the His-Ni complexes were much less taken up by vacuoles than free Ni ions

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This distinction is crucial: most fundamental studies on the actin mechanism focus on the entire T4, and extrapolation to the TB-500 fragment alone remains incomplete in the published literature