The GHK Tripeptide The GHK tripeptide consists of three amino acids: Glycine Histidine Lysine Although small, this peptide contains several potential metal-binding sites
152803 [online] Available at: Alber, J., Freisinger, P
Reactions probing the cleavage site were prepared by preparing reaction mixtures to contain 16 M and 8 M methyl green and netropsin respectively from stock solutions prepared in 80 mM HEPES buffer (pH = 7.4)
The two peptides hit different receptors and pathways, so there's no known direct interaction
Immunocytochemistry Cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) for 10 min at 20 C, washed three times with PBS, and then incubated with blocking buffer (1% FBS and 1% BSA in PBS) for 1 h at 20 C
Keep it in the refrigerator to keep it potent