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AOD-9604, a synthetic fragment derived from the C-terminal region of human growth hormone, has been subjected to extensive clinical evaluation involving over 900 participants across six randomized controlled trials

Exome sequencing of 37 samples from 16 PCa patients revealed recurrent alterations in DNA damage repair (DDR) genes, RTK/RAS pathway components, and autophagy regulators, with copy number variation burden correlating with metastatic potential (Wu et al., 2020)

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Then, the cells were washed and suspended in Roswell Park Memorial Institute-1640 medium (RPMI-1640, Sigma-Aldrich) supplemented with 2 g/L sodium bicarbonate, 10% fetal bovine serum (FBS, Sigma-Aldrich), 2% glutamine, and 100 U/mL penicillin-0.1 mg/mL streptomycin (Sigma-Aldrich) and incubated in vitro with RPMI medium (control), tert -Butyl hydroperoxide (tBHP: 200 M) or tBHP plus Ab (22.5 mg/mL) for 30 min at 37C