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The temperature and relative humidity of the cultivation environment were set at 25 C and 70%, respectively

reported that PAL mutants of Arabidopsis were more sensitive to ultraviolet light than wild type plants but were more tolerant to drought [51]

These can include better tissue repair and more energy

doi: 10.1016/j.bmc.2012.04.029 86

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In vitro PLA2G2F activity assay Sample preparation and in vitro reaction Control, PLA2G2F isoform 2- and PLA2G2F-H110Q-overexpressing RT112 cells were harvested from 85% confluent 15-cm plates in lysing buffer (50 mM Tris-HCl (pH 8.0), 150 mM sodium chloride (NaCl), 0.5% Triton X-100 and 5% glycerol (v/v)) containing protease inhibitor (Thermo Scientific, A32955)