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The total number of nuclei and the number of YAP-positive nuclei were quantified, and, subsequently, the number of YAP-negative nuclei was calculated by subtracting the number of YAP-positive nuclei from the total number of nuclei

H.LumsdenA

Cross-talk between hepatic stellate cells and T lymphocytes in liver fibrosis

Cysteine protease-associated DUBs include the ubiquitin carboxyl-terminal hydrolases (UCHs), ubiquitin specific proteases (USPs), Machado Joseph Diseases (MJD) proteases, and ovarian tumor (OTU) protease

Chauhan et al., 2012)

For long-term studies or to prevent degradation from repeated freeze-thaw cycles, it is best practice to aliquot the freshly reconstituted solution into single-use volumes and store them frozen at -20C or colder