Lysosomal accumulation was observed in SMCR8 deficient macrophages from Smcr8 /mice , possibly because of increased ROS (Mcalpine et al., 2018)
The altered autoregulation of blood flow cannot meet the metabolic demand of tissues in ME/CFS, leading to tissue hypoxia and subsequent ischaemia/reperfusion injury with its associated symptoms and signs ( Endothelial damage Endothelial cells are important regulators of the immune system (308)
Circular dichroism of proteins All the purified proteins were individually dialyzed at 4 C, checked for the GST tag using an anti-GST antibody, and CD spectra were measured (JASCO-CD Spectropolarimeter J-815 Serial no
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Amtul, Z., Uhrig, M., Wang, L., Rozmahel, R
Calculating the GSH/GSSG ratio from these measurements provides a reliable indicator of intracellular redox status in preclinical models