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( b ) Propionylcarnitine abundance in cells cultured in normal media or under glutamine starvation (GS) and supplemented with KG (n = 8 technical replicates representative from 3 independent experiments in each cell line pair)

The mix was incubated for 2 min and then 1 l of Thermoscript reverse transcriptase (200 U) (Invitrogen) was added and incubated for 50 min at 42 C

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doi: 10.1016/s0167-0115(96)02116-7