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But despite being one of the most commonly used laboratory reagents in peptide research, bacteriostatic water generates a steady stream of questions from Canadian researchers: what is it actually, does it need refrigeration, how long does it last, how does it differ from sterile water, can you use it for multiple peptides, and what happens if you use the wrong kind of water instead

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The Moment You Open the Vial: A Critical Juncture The second a needle punctures that rubber stopper, the sterile environment is technically breached

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Common reconstitution scenarios clinicians use: 50 mg vial + 2.5 ml water 20 mg/ml - 1 mg = 0.05 ml ( 5 units on a U-100 insulin syringe)