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the cultures were diluted with minimal growth medium (AB medium) supplemented with 0.5% glucose, 10% TSB, and 0.05 mM PI, to an OD 600 of 0.001

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All Adalya shisha contains nicotine, and users should be aware of the health risks associated with tobacco use.

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It has a push button opening and measures 10cm by 8.5cm when closed