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The supernatant was discarded and 5 mL of 50 mmol/L KH 2 PO 4 K 2 HPO 4 buffer solution with a pH of 7.5 was added to the remaining solution which was then ultrasonically crushed

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Novel high-throughput single-cell approaches, including RNA 171 and bisulphite sequencing 172 , as well as assay for transposase-accessible chromatin (ATAC) 173 and Cleavage Under Targets and Tagmentation (CUT&Tag) 174 allow transcriptomic and epigenomic profiling of low-input heterogeneous tissue at single-cell or -nucleus resolution

In previous years this would have destroyed the plant, however it has become possible to harvest the briar without destroying the shrub

In this example