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1 g/mL ionomycin and 100 ng/mL phorbol 12-myristate 13-acetate were added to the culture media with 0.01% Protein Transport Inhibitor (BD GolgiStopTM, BD biosciences, NJ, USA)

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Though the NFL used to suspend players for entire seasons for testing positive for marijuana just ask Ricky Williams the league has substantially loosened its pot policy over the past decade and changed its previous window of testing, which spanned from April to August, to the first two weeks of training camp