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Front cell Dev Biology 8:229 Espinosa-Leal CA, Puente-Garza CA, Garca-Lara S (2018) In vitro plant tissue culture: means for production of biological active compounds

Following an additional washing step with PBS, nonspecific binding sites were blocked with 1% bovine serum albumin (BSA) in PBS for 30 min at room temperature followed by staining with primary antibody against AIF (rabbit anti-AIF antibody, Cell Signaling, Danvers, MA, USA

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