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Characterisation of phenolic by HPLC (high-performance liquid chromatography) analysis The HPLC phenolic characterization was performed by separation module Waters 2690 Alliance (Waters, Rydamere, NSW, Australia), paired with Waters 2998 Photodiode Array (PDA) detector following published protocols with modifications 21 .The separation was executed using a Synergi Hydro-RP 80A, LC Column (250 mm 4.6 internal diameter, 4 m particle size) (Phenomenex Inc., Lane Cove West, NSW, Australia)

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ISSN 1941-0646

The brand offers both regular and light tobacco options