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This gene fragment was cloned in a pIDTblue T7 expression vector upstream of a shortened TMV sequence 1166 bp long (the full length TMV genome is 6,395 nt in length) containing the 234 bp-long OAS to generate the plasmid pIDTblue/EBOVL1-TMVshort (Supplementary Information, Fig

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The Wyoming Tobacco Prevention and Control Program (TPCP) shares four goals with the federal tobacco prevention and control program to reduce tobacco-related chronic disease, and death: (a) reduce tobacco initiation (CDC, 2014b), (b) decrease exposure to secondhand smoke (CDC, 2017), (c) increase tobacco cessation attempts and successes (CDC, 2015), and (d) eliminate tobacco-related disparities (CDC 2014b