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When nicotine is eliminated, there is a dopamine fall, which causes a loss of pleasure, low motivation, and depression, Dr

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Then further annotation was performed using SnpEff (version 5.0-1) software 26 to obtain detailed information and prediction of the gene in which the SNP is located, and the CDS sequence of the gene in which the SNP is located was placed on NCBI for comparison to further screen for genes highly associated with the plant

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