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For the TNBS-induced colitis model, approximately 5 10 6 Th17 cells from cigarette-exposed Gpr15 +/+ and Gpr15 / mice were transferred into Gpr15 / mice via tail vein injection on the third day of colitis induction, and the clinical signs of colitis were monitored

That figure is spread out across three cigarette brands, the only ones GTI has in its product portfolio at the moment: Cavallo, Milano, and Mond (see table)

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The resulting solution was regarded as 100 % CSE and, within 1 h, was diluted with medium for use in experiments

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