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It is generally accepted that in vitro studies with concentrations of active compounds beyond the IC50 concentration of 25 M for pure compounds and the IC50 of 100 g/mL for the extracts have no therapeutic significance because they could not be implemented in vivo and are not appropriate for further pharmaceutical development [272]

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This dual clinical-pharmacy formulation approach ensures that active ingredients are present at evidence-supported concentrations, with bioavailability considerations built into the sachet delivery format

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methodology, O.T., F.D