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(1) In spite of these guidelines, many tobacco barns continued to be constructed however the farmer saw fit

Functional validation included virus-induced gene silencing (VIGS) of homologous genes in Nicotiana benthamiana and exogenous application of synthetic NtPSY1/NtPSY3 peptides to assess their effects on seed germination, seedling growth, and osmotic stress tolerance

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In fact, in a 2018 Truth Initiative study of support for tobacco policies, residents of Tobacco Nation supported policies at almost exactly the same level as their counterparts in states outside Tobacco Nation