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Additionally, they did not assess the durations of the gastrointestinal conditions, so causal relationships between SHS with PUD and GERD could not be evaluated

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Gravimetric analysis was performed via A/E glass fiber filters (PALL Life Sciences, Tijuana, Mexico) to measure the concentration of total particulate matter (TPM), which was approximately 200 mg/m within a chamber containing six cigarettes

After the transfer, membranes were blocked for 1 h in TBST and 5% non-fat milk and then incubated overnight with the primary antibody at 4C