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Flow cytometry analysis For surface staining, the cells were incubated with antibodies, including anti-CD3-AF700, anti-CD4-FITC, anti-CD45-BV510, anti-CD8-Percp-cy5.5, anti-GPR15-APC, and anti-CD25-BV650 (all from eBioscience), for 30 min at 4 C in the dark