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The adolescent brain is particularly susceptible to the addicting effects of nicotine, and just raising the legal age to buy tobacco to 21 would help get kids past the most vulnerable point, he says

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Following three 10 min washes in PBS-T, the membrane was incubated with HRP conjugated secondary antibodies, goat anti-rabbit (Thermo Scientific, 656120, 1:5000 dilution), or goat anti-mouse IgG antibody (Thermo Scientific, 626520, 1:5000 dilution) in blocking buffer for 1 hour at room temperature

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