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Unlike traditional cigarettes that involve tobacco combustion, ECs use heat to transform the liquid (known as e-liquid) into an inhalable aerosol

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For phospho-protein staining, cells were treated with the indicated smoking-related stimuli for an appropriate amount of time, after which they were resuspended in 100 L of culture medium and fixed with an equal volume of BD Cytofix fixation buffer for 15 min at 37 C, resuspended in 150 L of BD Cytoperm Solution III for 30 min on ice, and resuspended in PBS containing 2% FBS and PE Mouse Anti-H2AX (pS139) (1:50) or PE Mouse anti-NF-B p65 (pS529) (1:50) for 20 min at room temperature

This reduces the blood's ability to carry oxygen to the body's tissues and organs, leading to oxygen deprivation

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