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Afterwards a 5-min suction phase followed in order to clean the air in the glass chamber for the next cycle

While some of the discrepancies in these results may be from our definition of M1 vs M2, which is a reductive approach to macrophage phenotyping, it is also likely that differences in dose, duration, constituent chemicals and other aspects of e-cigarette delivery are responsible, as well as altered cellular responses in the setting of signaling from other cells in vivo