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Preparation of Ce-DAB2-stained fly tissues for EM Fly brains and spinal cords were dissected according to previously described methods

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This has been approached using thiosugars and alkene-containing peptide chains as well as using alkene-functionalised sugars with native proteins

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Thus, we performed phasor fluorescence lifetime imaging (phasor-FLIM) to measure fluorescence lifetime ( f ), which can be correlated to the molecular self-assembly 21,34