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[28] noted that in Bhatta and Glantzs analysis [31], the majority of EC users currently or formerly smoked: only 12 participants out of 17,601 used ECs but had never smoked cigarettes, and none of these 12 developed respiratory symptoms in the PATH waves examined

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Moreover, a decreased expression of the epithelial marker E-cadherin and the tight junction protein zonula occludens-1 (ZO-1) was observed in A549 and H1650 cells exposed to e-liquid [26]