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cDNA was synthesized from RNA using Revert Aid 1st Strand cDNA synthesis kit (Thermo, USA), followed by real-time PCR analysis in a CFX96 real-time PCR instrument (Bio-Rad, Germany)

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One study found that smoking men had significantly higher levels of total testosterone compared to men who never smoked

Note: Cigarette-to-vape puff equivalency is an estimate

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