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Primary antibodies included anti RPS14 (Adcam), anti-RPL30 (Invitrogen), and anti-GAPDH (Santa Cruz Biotechnology)

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Two randomized trials examined individuals who switched from tobacco cigarettes to electronic cigarettes, revealing that using ECs for 5 days did not lead to negative respiratory health outcomes, with spirometry findings showing no significant effect on airflow obstruction or lung function after EC use (32)

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