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Systematic Climate Model Biases in the Large-Scale Patterns of Recent Sea-Surface Temperature and Sea-Level Pressure Change

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Materials and methods Vector construction and tobacco transformation To construct the NtGLK85 ( Nitab4.5_0010689g0010 ) overexpression vector (abbreviated as D710), the full-length cDNA of NtGLK85 was cloned from the cDNA of the tobacco variety K326 using GLK85-F/GLK85-R primers (Table S6) via PCR