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Cells were pre-washed in 0.1 M cacodylate buffer and post-fixed in 1% OsO4 supplemented with 1.5% K3Fe(Cn6) for 2 hours, dehydrated and embedded in epoxy resin according to routine procedures

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However Sarri isnt the only Italian manager who loves his cigarettes, here are other three of his other managerial counterparts that love to light up