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Most cells are capable of glutathione synthesis especially hepatocytes since glutathione reductase enzyme is present in these cells

Studies have found that the longer cellular damage is allowed to occur, the more cellular function is impaired, giving way to the accumulation of even more damaged cells and accelerated aging

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Metabolic activation of unsaturated derivatives of valproic acid

*Correspondence: Ming Li, [email protected] Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

2) Resuspend the cells in 10 mL of Equilibration buffer (LE buffer) with appropriate amount of PMSF (Final working concentration can be 1mM) and add lysozyme (Final working concentration is 0.2-0.4 mg/mL), or other protease inhibitors cocktail added