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Tympanic bullae were harvested and fixed in 4% paraformaldehyde (P2031, Biosesang, Seongnam-si, Republic of Korea) for 24 h at 4 C and rinsed with PBS and decalcified in 10% ethylenediaminetetraacetic acid (EDTA

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Both current and ever e-cigarette users exhibited lower scores in principal component-1 (perception of harm) compared with their counterparts, while scoring higher in principal component-2 (perception of benefit)

This is because the process in which they are prepared is different