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Rapid Prototyping Enzyme Homologs to Improve Titer of Nicotinamide Mononucleotide Using a Strategy Combining Cell-free Protein Synthesis with Split GFP

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Singh Raghav KP, Xiao L, Lewis C, Zeller BE, Overman MJ, Huey RW, Dasari A, Chang K, Quinn K, YC Shen JP, Parseghian CM, Willis J, Ludford K, Van K Morris II, Wolff RA, Wang XS, Drusbosky L, Kopetz S

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Furthermore, we found that chronic hypoxia enhanced ceramide catabolism by inducing hypoxia-inducible factor (HIF)-2 and HIF-2-dependent transcriptional activation of alkaline ceramidase 2 (Acer2)