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Oxygen consumption rate (OCR) was measured using a Seahorse XFe24 Analyzer (Agilent Technologies) under basal conditions and following sequential injection of oligomycin, FCCP, and rotenone/antimycin A

Amycretin is a unimolecular dual agonist -- a single molecule that activates both GLP-1 and amylin receptors -- available in both injectable and oral formulations

The decrease in complex I activity drives an increase in glycolysis which in turn results in increased lactate production in intestinal epithelial cells and, in turn, increased Lac-Phe synthesis