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Such lighters are often far hotter than normal lighters (those that use a "soft flame") and can burn in excess of 1,100 C (2,010 F)

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Membranes were treated with 5% BSA in PBS-T (PBS with 0.1% Tween 20) blocking buffer for at least 1 h at room temperature, then incubated with primary antibody diluted in blocking buffer overnight at 4C