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Doi: 10.1053/j.seminoncol.2004.11.011

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After replacing the medium with fresh live imaging buffer, images were acquired using a 40 objective lens on an Olympus FLUOVIEW FV3000 confocal laser scanning microscope (Olympus Co., Tokyo, Japan)

The term symptom is also avoided, as it is very clinical and can imply that something is wrong and needs fixing