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b Chi-squared test, for contingencies testing the differences in the characteristics of participants between Wave 1 and Wave 2

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E-cig device and e-liquids The eVic Supreme with a Delta 23 atomizer (Joyetech, Shenzen, China) was used to generate e-cig aerosols for all experiments as previously described 11,19,20

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After evaluating the amplified products on 2% w/v agarose gel, the products were purified using Ampure XP kit (Beckman Coulter, Brea, CA, USA)