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The 30 l PCR reactions (with 2 l cDNA, 0.5 l (10 M) forward and 0.5 l (10 M) reverse primers, 15 l SYBR green, and 12 l PCR H2O) were undergone 10 min at 95 C, then 40 cycles of 15 s at 95 C and 60 s at 60 C

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