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To optimize SeNPs concentration for subsequent semen cryopreservation, three semen samples were individually split into four equals and diluted to a final concentration of 6.0 10 7 spermatozoa/ml with the basic extender supplemented with 0 g/ml SeNPs, 1 g/ml SeNPs, 2 g/ml SeNPs, and 4 g/ml SeNPs

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Under hypoxic conditions, lactate could enter fibrogenic mesenchymal progenitor cells through the GPR81 receptor to enhance their self-renewal, intensified by HIF-1 augmenting GPR81 expression [180]

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