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1 g/mL ionomycin and 100 ng/mL phorbol 12-myristate 13-acetate were added to the culture media with 0.01% Protein Transport Inhibitor (BD GolgiStopTM, BD biosciences, NJ, USA)

Research interest grew substantially beginning in 2003, with annual publications reaching 39 by 2014

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Known as thirdhand smoke, it settles like dust on hard surfaces and penetrates deep into soft surfaces like upholstered furniture