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A study conducted by Anzengruber et al
When school is disrupted, coordinated support matters, said DCYF Director Sherrice Dorsey-Smith
In this new regular series, AP is delving into the medicine catalogues of pharmacists of days past, to unearth often surprising remedies, like selling cigarettes for asthmatic children
Trends in antifungal drug resistance
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You end up with: 60-90% cleaved protein 10-40% uncleaved protein Same affinity tag on both Nearly identical size Why this is a nightmare: If cleavage is 80% complete: Re-running Ni-NTA removes 20% of your protein (the uncleaved fraction) But 80% of your cleaved protein also binds (weakly) due to native histidines You lose most of your cleaved protein trying to remove the uncleaved fraction The numbers: Studies report that His-tag cleavage is often "far from 100% efficient," with many proteins showing substantial uncleaved fractions even after optimization
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