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Types of Samples Cotinine can be measured in several biological specimens: Testing Methods Immunoassays (ELISA, rapid tests) Quick, inexpensive Can be qualitative (yes/no) or semi-quantitative Common in clinical and workplace screening Chromatography-based methods (GC-MS, LC-MS/MS) More accurate and quantitative Can distinguish nicotine from cotinine and other metabolites Used in research or forensic settings Interpretation of Results Cutoff values vary by lab and context
The remaining supernatant was purified on two solid-phase extraction (SPE) columns for hormones, following the protocol described by Schfer and colleagues (Schfer et al., 2016)
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