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Afterward, the obtained sperm pellets were treated with somatic lysis buffer (SLB: 0.1% SDS, 0.5% Triton-X-100 in DEPC water) on ice for 20 min and then centrifuged at 1000 g for 5 min

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It is understood that this trait is a measurable and heritable phenotype and hope this note encourages further research on the effect of genetic and environmental factors of DMSp formation in relation to other proteins and peptides

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