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To evaluate sequencing depth, dilution curves were plotted using R software (version 2.15.3)

82% of middle school students

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We opted for a single-respondent design, conducting repeated in-depth interview sessions with a carefully selected informant

The end of tobacco harvest looks quite different from the beginning, the first two passes during harvest only take four to five leaves off each plant, but the final pass takes the stalk and upper leaves the parts of tobacco with the highest value