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Rayens MK, Hahn EJ, Hedgecock S

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Cell transfection and lentiviral transduction for CRISPR knockout cells Plasmids containing enhanced specificity Cas9 and the appropriate small guide RNAs (sgRNA) in the pLentiCRISPRv2 vector were purchased from GenScript (Piscataway, NJ, USA) for generating stable knockout with lentivirus and selected using puromycin

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