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The lethal dose of nicotine for an adult is estimated at 3060 mg [52]

It has been reported that adult Drosophila lose their negative geotaxis reflex for a period of time that is proportional to the amount of the vaporized nicotine administrated (Sanchez-Diaz et al., 2015)

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Blood and bronchoalveolar lavage fluid (BALF) were collected post-exposure for immune cell analysis