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These 227 participants were then split into four groups based on their behavior: people who are light dual users, predominantly vapers, heavy dual users, and predominantly smokers

Post-induction, cells were harvested, sonicated, and the supernatant was subjected to Ni 2+ -affinity chromatography (HiTrap IMAC FF, Cytiva)

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in chewing and smokeless tobacco by microbial and molecular methods

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