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Tympanic bullae were harvested and fixed in 4% paraformaldehyde (P2031, Biosesang, Seongnam-si, Republic of Korea) for 24 h at 4 C and rinsed with PBS and decalcified in 10% ethylenediaminetetraacetic acid (EDTA

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3d), with a shift to lower values in the skin of smokers suggesting more compact packing of the predominant all trans chains

Additionally, smoking accelerates the ageing process, causing premature wrinkles and age spots, which can contribute to an overall darker or more uneven skin tone

No significant changes were observed for PSS-10, FFA, L-1, and SF12 MH scores from baseline to 12 weeks (see Table 8)